Keio Univ. Keio Univ.



研究業績

国際原著 Takae S., Tsukada K., Maeda I., Sato Y., Okamoto N., Kondo H., Motani Y., Suzuki N., Preliminary clinical study of optical coherence tomography intended for investigating effective ovarian tissue transplantation, Journal of Assisted Reproduction and Genetics, 35(4), 627-636, 2018.
https://doi.org/10.1007/s10815-018-1166-9
Hase T., Ishigaki S., Shibusawa K., Hamanaka S., Yabuki Y., Tamano Y., Tsukada K., Immobilized monolayer nanoparticles in a microfluidic device for surface enhanced Raman scattering measurement, Advanced Biomedical Engineering, 6, 122-128, 2018.
https://doi.org/10.14326/abe.6.122
Park J.Y., Kyung K.H., Tsukada K., Kim S.H., Shiratori S., Biodegradable polycaprolactone nanofibres with β-chitosan and calcium carbonate produce a hemostatic effect, Polymer, 123, 194-202, 2017.
https://doi.org/10.1016/j.polymer.2017.07.013
Morse K., Kimizuka Y., Chan M.P.K., Shibata M., Shimaoka Y., Takeuchi S., Forbes B., Nirschl C.J., Li B., Zeng Y., Bronson R., Katagiri W., Shigeta A., Sirbulescu R.F., Chen H., Tan R.Y.Y., Tsukada K., Brauns T., Gelfand J.A., Sluder A., Locascio J.J., Poznansky M.C., Anandasabapathy N., Kashiwagi S., Near-infrared 1064 nm laser modulates migratory dendritic cells to adjuvant the immune response to intradermal influenza vaccine, The Journal of Immunology, 199(4), 1319-1332, 2017.
https://doi.org/10.4049/jimmunol.1601873
Kimizuka Y., Callahan J.J., Huang Z., Morse K., Katagiri W., Shigeta A., Bronson R., Takeuchi S., Shimaoka Y., Chan M.P.K., Zeng Y., Li B., Chen H., Tan R.Y.Y., Dwyer C., Mulley T., Leblanc P., Goudie C., Gelfand J., Tsukada K., Brauns T., Poznansky M.C., Bean D., Kashiwagi S., Semiconductor diode laser device adjuvanting intradermal vaccine, Vaccine, 35, 2404-12, 2017.
https://doi.org/10.1016/j.vaccine.2017.03.036
Takae S., Tsukada K., Sato Y., Okamoto N., Kawahara T., Suzuki N., Accuracy and safety verification of ovarian reserve assessment technique for ovarian tissue transplantation using optical coherence tomography in mice ovary, Scientific Reports, 7, 43550 (8 pages), 2017.
https://doi.org/10.1038/srep43550
Oda K., Iwamoto Y., Tsukada K., Simultaneous mapping of unevenly distributed tissue hypoxia and vessel permeability in tumor microenvironment, Biomedical Physics & Engineering Express, 2, 065017, 2016.
https://doi.org/10.1088/2057-1976/aa5193
Ishikawa M., Kajimura M., Morikawa T., Tsukada K., Tsuji T., Kusaka G., Tanaka Y., Suematsu M., Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy, Journal of the Neurological Sciences, 368, 326-33, 2016.
https://doi.org/10.1016/j.jns.2016.06.067
Nishioka S., Tenjimbayashi M., Manabe K., Matsubayashi T., Suwabe K., Tsukada K., Shiratori S., Facile design of plant-oil-infused fine surface asperity for transparent blood-repelling endoscope lens, RSC Advances, 6, 47579-87, 2016.
Ishikawa M., Kajimura M., Morikawa T., Tsukada K., Tsuji T., Kusaka G., Tanaka Y., Suematsu M., Leukocyte plugging and cortical capillary flow after subarachnoid hemorrhage, Acta Neurochirurgica, 158(6), 1057-67, 2016.
Sato A., Kadokura K., Uchida H., Tsukada K., An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice, Biochemical and Biophysical Research Communications, 453, 767-71, 2014.
Yamada R., Tabuchi S., Tsukada, K., Optical scanning of tissue oxygen tension and hypoxia imaging in solid tumors, Advanced Biomedical Engineering, 3, 65-71, 2014.
Takada Y., Otsuka R., Tsukada K., A carbon monoxide sensing film based on hemoglobin allostery, Journal of Biomedical Science and Engineering, 7, 173-180, 2014.
Kashiwagi S., Yuan J., Forbes B., Hibert M.L., Lee E.L., Whicher L., Goudie C., Yang Y., Chen T., Edelblute B., Collette B., Edington L., Trussler J., Nezivar J., Leblanc P., Bronson R., Tsukada K., Suematsu M., Dover J., Brauns T., Gelfand J., Poznansky M.C., Near-infrared laser adjuvant for influenza vaccine, PLoS ONE, 8(12), e82899, 2013.
Uchida H., Sato A., Miyayama A., Tsukada K., Generation of an oxygen gradient in a microfluidic device and cellular analysis in hypoxia, Advanced Biomedical Engineering, 2, 143-149, 2013.
Tsukada K., Tajima T., Hori S., Matsuura T., Johnson R.S., Goda N. and Suematsu M., Hypoxia-inducible factor-1 is a determinant of lobular structure and oxygen consumption in the liver, Microcirculation, 20(5), 385-93, 2013.
Tsukada K. and Suematsu M., Visualization and analysis of blood flow and oxygen consumption in hepatic microcirculation: application to an acute hepatitis model, Journal of Visualized Experiments, 66, e3996. doi:10.3791/3996 (5 pages), 2012.
Shiwa T., Uchida H. and Tsukada K., Co-culture microdevice with oxygen gradient for tumor microenvironment model and metastasis imaging, Am. J. Biomed. Eng., 2(4), 175-180, 2012.
Handa K., Ohmura M., Nishime C., Hishiki T., Nagahata Y., Kawai K., Suemizu H., Nakamura M., Wakui M., Kitagawa Y., Suematsu M. and Tsukada K., Phosphorescence assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice, Advances in Experimental Medicine and Biology, 662, 423-9, 2010.
Akatsu T., Tsukada K., Hishiki T., Suganuma K., Tanabe M., Shimazu M., Kitagawa Y., Yachie-Kinoshita A. and Suematsu M., T-state stabilization of hemoglobin by nitric oxide to form a-nitrosyl heme causes constitutive release of ATP from human erythrocytes, Advances in Experimental Medicine and Biology, 662, 109-14, 2010.
Kondo K., Sugioka T., Tsukada K., Aizawa M., Takizawa M., Shimizu K., Morimoto M., Suematsu M. and Goda N., Fenofibrate, a peroxisome proliferator-activated receptor _ agonist, improves hepatic microcirculatory patency and oxygen availability in a high-fat-diet-induced fatty liver in mice, Advances in Experimental Medicine and Biology, 662, 77-82, 2010.
Kashiwagi S., Tsukada K., Xu L., Miyazaki J., Kozin S.V., Tyrrell J.A., Sessa W.C., Gerweck L.E., Jain R.K., Fukumura D., Perivascular nitric oxide gradients normalize tumor vasculature, Nature Medicine, 14(3), 255-7, 2008.
Kinoshita A., Tsukada K., Soga T., Hishiki T., Ueno Y., Nakayama Y., Tomita M., Suematsu M., Roles of hemoglobin allostery in hypoxia-induced metabolic alterations in erythrocytes: simulation and its verification by metabolome analysis, Journal of Biological Chemistry, 282(14), 10731-41, 2007.
Tsukada K.*, Suganuma K.*, Kashiba M., Tsuneshige A., Goda N., Kitajima M., Yonetani T., Suematsu M. (*equally contributed): Erythrocytes with T-state-stabilized hemoglobin as a therapeutic tool for post-ischemic liver dysfunction, Antioxidants & Redox Signaling, 8, 1847-55, 2006.
Suematsu M., Tsukada K., Tajima T., Yamamoto T., Ochiai D., Watanabe H., Yoshimura Y., Goda N.: Carbon monoxide as a guardian against hepatobiliary dysfunction, Alcoholism, clinical and experimental research, 29 (11), 134S-139S, 2005.
Tsukada K., Sekizuka E., Oshio C., Tsujioka K., Minamitani H.: Red blood cell velocity and oxygen tension measurement in cerebral microvessels by double-wavelength photoexcitation, Journal of Applied Physiology, 96(4), 1561-8, 2004.
Tsukada K., Sakai S., Hase K., Minamitani H.: Development of catheter-type optical oxygen sensor and applications to bioinstrumentation, Biosensors and Bioelectronics, 18(12), pp 1439-1445, 2003.
Minamitani H., Tsukada K., Sekizuka E. and Oshio C.: Imaging and functional analysis of blood flow in organic microcirculation, Journal of Pharmacological Science, 93(3), pp.227-33, 2003.
Ishikawa M., Sekizuka E., Oshio C., Sato S., Yamaguchi N., Terao S., Tsukada K., Minamitani H. and Kawase T.: Platelet adhesion and arteriolar dilation during photothrombosis -observation with rat closed cranial and spinal windows-, Journal of Neurological Science, 194(1), pp. 59-69, 2002.
Tsukada K., Sekizuka E., Oshio C., Minamitani H.: Direct measurement of erythrocyte deformability in diabetes mellitus with transparent microchannel capillary model and high-speed video camera system, Microvascular Research, 61(3), pp 231-9, 2001.
Tsukada K., Minamitani H., Sekizuka E., Oshio C.: Image correlation method for measuring blood flow velocity in microcirculation: correlation 'window' simulation and in vivo image analysis., Physiological Measurement, 21(4), pp 459-71, 2000.

邦文原著 山田 遼,堀之内宏久,塚田孝祐:腫瘍の低酸素イメージングと酸素化過程における時空間解析,電気学会論文誌C(電子・情報・システム部門誌),132(10), 1602-7, 2012.
南谷晴之,川村友美,塚田孝祐,飯島淳彦,関塚永一,大塩 力:原子間力顕微鏡による赤血球弾性の計測,電気学会論文誌C(電子・情報・システム部門誌),122-C(9), pp. 1664-71, 2002.
塚田孝祐,長谷憲多朗,関塚永一,大塩 力,南谷晴之:光励起による臓器微小循環の血流動態および酸素分圧計測,電気学会論文誌C(電子・情報・システム部門誌),121-C(9), pp. 1415-21, 2001.

著  書 塚田孝祐:低酸素のバイオイメージング,別冊医学のあゆみ「レドックス ~ストレス防御の医学~」分担執筆, pp. 183-7, 2005.

解説論文 塚田孝祐,“標識しない”という新たなバイオイメージングの流れ, 光学, 44(6), pp. 215, 2015.
塚田孝祐, 福村 大, NOによる腫瘍血管構造の正常化と放射線治療, 血液・腫瘍科, 57(6), pp. 652-7, 2008.
末松 誠,菱木貴子,岩渕拓也,山本雄広,行武良哲,塚田孝祐:メタボローム解析技術による新しい代謝制御機構の系統的探索:ガスバイオロジーの推進と医学への応用,細胞工学,25(12),pp.1427-31,2006.
南谷晴之,塚田孝祐:臓器血流と酸素代謝の光・イメージング解析,医学のあゆみ,210(3), pp. 200-204, 2004.
塚田孝祐,酒井修平,長谷憲多朗,南谷晴之:医用カテーテル型光学式酸素センサ,医工学治療,14(3),pp. 163-167,2002.

国際会議 Fujioka Y., Katayama Y., Tsukada K., Development of Flexible Oxygen Sensor for Direct Measurement of Vital Oxygen Partial Pressure, IEEE Life Sciences Conference (LSC), 2018-10, (Montreal, Canada).
Takae S., Tsukada K., Sato Y., Okamoto N., Nishijima C., Suzuki Y., Yoshioka N., Sugishita Y., Horage U., Kondo H., Kawamura K., Suzuki N., Quantification and localization of primordial follicle using optical coherence tomography intended for investigating effective ovarian tissue transplantation, 8th Congress of the Asia Pacific Initiative on Reproduction, 2018-4, (Taipei, Taiwan).
Iwamoto Y., Oda K., Tsukada K., Quantification and imaging of vascular permeability and partial pressure of oxygen in vivo, 2017 Annual Meeting of BMES, 2017-10, (Phoenix, USA).
Katagiri W., Tsukada K., Three-dimensional in vivo analysis of histology for low-level laser therapy, 2017 Annual Meeting of BMES, 2017-10, (Phoenix, USA).
Takae S., Tsukada K., Okamoto N., Sato Y., Kawahara T., Suzuki N., Verification of accuracy & safety for ovarian reserve assessment with optical coherence tomography using mouse ovary, The ASRM Annual Meeting 2016, 2016-10, (Salt Lake City, USA).
Park J., Kyung K., Kim S., Tsukada K., Shiratori S., Biodegradable nanofiber with beta-chitosan and calcium carbonate for hemostatic effect, Biomaterials International 2016, 2016-10, (Kenting, Taiwan).
Boudehent B., Kadokura K. and Tsukada K., Quantitative analysis of HUVEC tube formation in culture under an oxygen gradient, 2016 Annual Meeting of BMES, 2016-10, (Minneapolis, USA).
Kadokura K., Sato A., Boudehent B. and Tsukada K., A cell culture device with continuous oxygen gradient for microcirculation research in vitro, 2016 Annual Meeting of BMES, 2016-10, (Minneapolis, USA).
Shiratori S., Nishioka S., Sasaki K., Tenjimbayashi M. and Tsukada K., Durable blood-repelling surface coating for medical application, Biomaterials International 2016, 2016-8, (Kenting, Taiwan).
Nishioka S., Tenjinbayashi M., Tsukada K. and Shiratori S., Anti-biofouling surface with plant oil infused porous structure for laparoscope lenses, The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015-12, (Hawaii, USA).
Kadokura K., Sato A., Okazaki Y. and Tsukada K., A cell culture microdevice with a continuous oxygen gradient for microvascular research in vitro, 10th World Congress for Microcirculation, 2015-9, (Kyoto, Japan).
Oda K., and Tsukada K., Quantification and imaging of regional vascular permeability and partial pressure oxygen, 10th World Congress for Microcirculation, 2015-9, (Kyoto, Japan).
Kadokura K., Sato A., Uchida H. and Tsukada K., Angiogenesis imaging in a cell culture microfluidic device mimicking the tumor microenvironment, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2015-8, (Milano, Italy).
Hase T., Tamano Y. and Tsukada K., Direct measurement of glycolytic metabolites with nanoparticle-based surface-enhanced Raman scattering, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2015-8, (Milano, Italy).
Ando Y., Yanagisawa Y. and Tsukada K., An organic light-emitting diode for oxygen sensing based on phosphorescence lifetime, 2014 Annual Meeting of BMES, 2014-10, (San Antonio, USA).
Morse K., Yuan J., Forbes B.M., Hibert M.L., Lee E.L.Q., Tan R.Y.Y., Whicher L., Goudie C., Yang Y., Chen T., Edelblute B., Collette B., Edington L., Trussler J., Nezivar J., Leblanc P., Bronson R., Tsukada K., Suematsu M., Dover J., Brauns t., Gelfand J., Poznansky M.C., Kashiwagi S., Near-Infrared Lasers to Adjuvant Vaccines, Infectious Disease World Summit, 2014-7, (Boston, USA).
Tsukada K., Laser-assisted hypoxia imaging for tumor biology, 37th Naito Conference, 2014-7, (Niseko, Japan).
Sato A., Uchida H., Miyayama A. and Tsukada K., Development of a microfluidic device forming oxygen gradient for cellular experiments, 26th International Microprocesses and Nanotechnology Conference (MNC 2013), 2013-11, (Sapporo, Japan).
Tomita K. and Tsukada K., An identification system for unstained cells using Mie scattering, 2013 Annual Meeting of BMES, 2013-9, (Seattle, USA).
Miyayama A., Uchida H., Sato A. and Tsukada K., Development of oxygen-sensing films and their application in biometry, 2013 Annual Meeting of BMES, 2013-9, (Seattle, USA).
Tsukada K., Generation of oxygen gradients in microfabricated devices for hypoxia research in vitro, Collaborative Conference on Materials Research (CCMR) 2013, 2013-6, (Jeju, Korea).
Osaki H., Tamano Y. and Tsukada K., Fluorescence reduction in time-resolved Raman spectroscopy for the detection of circulating tumor cells, 2012 Annual Meeting of BMES, 2012-10, (Atlanta, USA).
Yoshida R., Yanagisawa Y. and Tsukada K., Development of a phosphorescence-based oxygen sensor using organic electroluminescence, 2012 Annual Meeting of BMES, 2012-10, (Atlanta, USA).
Uchida H., Shiwa T., Sato A. and Tsukada K., Development of a microfluidic device forming oxygen gradient for cell culture, 2012 Annual Meeting of BMES, 2012-10, (Atlanta, USA).
Yamada R., Horinouchi H. and Tsukada K., Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2011-9, (Boston, USA).
Sasaki N., Horinouchi H., Ushiyama A., Tsukada K. and Minamitani H., Oxygen diffusion constant and oxygen consumption in arteriolar wall in vivo, International Society for Oxygen Transport to Tissues 2009-7, (Cleveland, USA).
Tsukada K., Conner D., Lin Q., Kucherlapati R., Jain RK and Fukumura D., Blockade of inducible nitric oxide synthase normalizes murine breast cancer vessels, 100th American Association for Cancer Research, 2009-4, (Denver, USA).
Handa K., Ohmura M., Nishime C., Hishiki T., Nagahata Y., Kawai K., Suemizu H., Nakamura M., Wakui M., Kitagawa Y., Suematsu M. and Tsukada K., Phosphorescence assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice, International Society for Oxygen Transport to Tissues 2008, (Sapporo, Japan).
Akatsu T., Tsukada K., Hishiki T., Suganuma K., Tanabe M., Shimazu M., Kitagawa Y., Yachie-Kinoshita A. and Suematsu M., T-state stabilization of hemoglobin by nitric oxide to form a-nitrosyl heme causes constitutive release of ATP from human erythrocytes, International Society for Oxygen Transport to Tissues 2008, (Sapporo, Japan).
Kondo K., Sugioka T., Tsukada K., Aizawa M., Takizawa M., Morimoto M., Suematsu M., Goda N.: Fenofibrate, a peroxisome proliferator-activated receptor a agonist, improves hepatic microcirculatory patency and oxygen availability in a high-fat diet-induced fatty liver in mice, International Society for Oxygen Transport to Tissues 2008, (Sapporo, Japan).
Kinoshita A., Tsukada K., Soga T., Hishiki T., Tomita M. and Suematsu M.: Phosphofructokinase is critical for hypoxia-induced activation of glycolysis in human erythrocytes: Prediction by simulation and verification by metabolome analysis, 8th World Congress for Microcirculation, 2007-8, (Milwaukee, USA).
Tsukada K., Tajima T., Kawamura T., Goda N., Johnson R., Suematsu M.: Hypoxia inducible factor-1 modulates oxygen consumption in hepatocytes, American Society for Cell Biology 46th Annual Meeting, 2006-12, (San Diego, USA).
Tsukada K., Tajima T., Kawamura T., Goda N., Johnson R., Suematsu M.: Increased oxygen consumption in hepatocyte-specific HIF-1α-targeted mice, 24th European Conference on Microcirculation, 2006-8, (Amsterdam).
Tsukada K., Tajima T., Goda N., Johnson R., Suematsu M.: HIF-1 as a modulator of oxygen consumption in hepatocytes, 20th international congress of biochemistry and molecular biology, 2006-6, (Kyoto).
Tsukada K., Tajima T., Goda N., Soga T., Johnson R., Suematsu M.: Hepatocyte-specific gene targeting of hypoxia-inducible factor-1 blunts hepatic regeneration and remodeling of oxygen metabolism after partial hepatectomy, 4th HO conference, 2005-10, (Boston).
Akatsu T., Kajimura M., Tsukada K., Mizuno R., Ohhashi T., Suematsu M.: Involvement of erythrocyte in hypoxic vasodialtion: role of ATP from the cell. The 30th Annual Meeting of The Japanese Society for Microcirculation and 6th Asian Congress for Microcirculation, 2005-2, (Tokyo).
Tsukada K., Suganuma K., Yonetani T., Suematsu M.: T-state stabilization of hemoglobin allostery: A potential therapeutic stratagem for post-ischemic organ microvascular damages, 6th Asian Congress for Microcirculation, 2005-2, (Tokyo).
Arai T., Tsukada K., Sekizuka E., Oshio C., Terao S., Hase K., Minamitani H., Effect of hemodilution on oxygen supply to brain cortical arterioles, 25th Annual International coference of the IEEE-EMBS and Annual Fall Meeting of the BMES, 2003-9, (Cancun).
Hase K., Sakai S., Tsukada K., Sekizuka E., Oshio C., Minamitani H., Continous measurement of blood oxygen pressure using a fiber optic sensor based on phosphorescence quenching, 24th Annual International coference of the IEEE-EMBS and Annual Fall Meeting of the BMES, 2002-10, (Houston).
Minamitani H., Kawamura T., Sekizuka E., Tsukada K. and Oshio C., Viscoelasticity of diabetic erythrocytes measured by microscopic flow imager and atomic force microscope, The 7th world congress for microcirculation, 2001-8, (Sydney).
Tsukada K., Hase K., Sekizuka E., Oshio C. and Minamitani H., Blood flow imaging and oxygen measurement by photoexcitation during haemorragic shock, The 7th world congress for microcirculation, 2001-8, (Sydney)
Minamitani H., Tsukada K., Kawamura T., Sekizuka E. and Oshio C., Analysis of elasticity and deformability of erythrocytes using micro-channel flow system and atomic force microscope, 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine & Biology, 2000-10. (Lyon).
Tsukada K., Hase K., Sekizuka E., Oshio C and Minamitani H., Simultaneous measurement of blood flow distribution and oxygen tension by photo-excitation in organ microcirculation, 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine & Biology, 2000-10. (Lyon).
Minamitani H., Kawamura T., Sekizuka E., Shimizu T, Tsukada K. and Oshio C., Deformability and viscoelasticity of diabetic erythrocytes measured by microchannel flow system and atomic force microscope, 21st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1999 (Atlanta).
Nagai, T., Sekizuka E., Tsukada K., Shimizu T., Minamitani H., Hokari, R., Oshio C., Effects of aldose reductase inhibitor in diabetic rats, 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1998 (Hong Kong)
Tsukada K., Sekizuka E., Oshio C., Nagai T. and Minamitani H., Capillary model of crystal microchannel for red blood cell deformability measurement, 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1997 (Chicago).
Tsukada K., Minamitani H., Sekizuka E. and Oshio C., Blood flow measurement system for microcirculation by image correlation method, 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1996 (Amsterdam).